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Table 3. Effect of KCl concentration on the growth of P. chrysogenum transformants obtained by two methods.
|
The average diameter of colonies (mm) |
The concentration of KCl (mol/L) |
||||||||||
|
0 |
0.5 |
0.9 |
1.3 |
1.7 |
2.1 |
2.5 |
2.9 |
3.3 |
3.7 |
4.1 |
|
|
Control |
>7.5 |
>7.5 |
5-7.5 |
<5 |
- |
- |
- |
- |
- |
- |
- |
|
Transformants-p |
- |
- |
<5 |
5-7.5 |
>7.5 |
>7.5 |
>7.5 |
>7.5 |
>7.5 |
>7.5 |
>7.5 |
|
Transformants-a |
>7.5 |
>7.5 |
5-7.5 |
<5 |
- |
- |
- |
- |
- |
- |
- |
|
Note: the diameter of
colony >7.5mm, good growth; 7.5mm>the diameter of colony<5mm
, poor growth; -, no growth; Transformants-p: P. chrysogenum transformants
by transformation of protoplasts with PEG; Transformants-a: P. chrysogenum
transformants using A. tumefaciens; twenty strains were selected from
control, transformants-p, transformants-a, respectively.
|
|
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