|
|
Table 2. Effects
of protoplasts prepared from per 100 mg mycelia collected at different
growth time on the efficiencies of transformation.
|
|
The concentration of protoplasts |
The growth time of mycelia for the preparation of protoplasts (hours) |
||||
|
0 |
24 |
36 |
48 |
60 |
|
|
1×106/ml |
- |
29 |
117 |
238 |
209 |
|
1×107/ml |
- |
25 |
145 |
255 |
213 |
|
1×108/ml |
- |
27 |
108 |
220 |
217 |
|
|