Figure 2.

a. Electrophoretogram of agarose gel
b. Southern blot

A: marker;
B: control P. chrysogenum genomic DNA;
C: digestion of control P. chrysogenum genomic DNA by Cla I;
D: transformant-p F5 genomic DNA;
E: digestion of transformant-p F5 genomic DNA by Cla I;
F: digestion of pYG715vgb by Cla I;
G: transformant-a F5 genomic DNA;
H: digestion of transformant-a F5 genomic DNA by Cla I;
I: digestion of pBI121vgb+ by Cla I;
transformant-p: P. chrysogenum transformant by PEG transformation of protoplasts;
transformant-a: P. chrysogenum transformant using A. tumefaciens.


Supported by UNESCO / MIRCEN network