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Table 2. Sequences of AFLP primers and adapters used to evaluate genetic diversity in Uruguayan weedy and cultivated rice.
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| Eco RI adapter: | 5'-CTGCTAGACTGCGTACC-3' 3'-CTGACGCATGGTTAA- 5' |
| Mse I adapter: | 5'-GACGATGAGTCCTGAG-3' 3'-TACTCAGGACTCAT-5' |
| Pre- Selective primers: | |
| E00 primer (+ 0 nt.) | 5'-GACTGCGTACCAATTC- 3' |
| M00 primer (+0 nt.) | 5'-GATGAGTCCTGAGTAA- 3' |
| Selective primers: | |
| E12 primer ( + 2 nt.) | 5'-GACTGCGTACCAATTCAC- 3' |
| E32 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCAAC-3' |
| E33 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCAAG-3' |
| E39 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCAGA- 3' |
| E42 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCAGT- 3' |
| E44 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCATC-3' |
| E47 primer (+ 3 nt.) | 5'-GACTGCGTACCAATTCCAA-3' |
| E58 primer (+ 3nt.) | 5'-GACTGCGTACCAATTCCGT-3' |
| M33 primer (+ 3 nt.) | 5'-GATGAGTCCTGAGTAAAAG-3' |
| M39 primer (+ 3 nt.) | 5'-GATGAGTCCTGAGTAAAGA-3' |
| M41 primer (+ 3 nt.) | 5'-GATGAGTCCTGAGTAAAGG-3' |
| M47 primer (+ 3 nt.) | 5'-GATGAGTCCTGAGTAACAA-3' |
| M64 primer (+ 3 nt.) | 5'-GATGAGTCCTGAGTAAGAC-3' |
| + 0 nt. = sequence of primer with no extra
nucleotides. + 2 nt. = 2 extra nucleotides added to the former primer sequence + 3 nt. = 3 extra nucleotides added to the former primer sequence. Underlined sequence = EcoRI and Mse adapter sequences included in each primer respectively. |
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