Table 1 - Immobilization and stabilization of papain on chelating sepharose: a metal chelate regenerable carrier

Table 1. Immobilization and reimmobilization of papain on chelating Sepharose activated by Cu²⁺ ions. Results represent the mean and standard deviation of three different immobilized preparations. Activity and immobilization yields were calculated from mean values.

No. of cycles	Enzyme activity (Units / mL gel)			Activity Yield (%) C/A x 100	Immobilization Yield (%) (A-B)/A x 100
No. of cycles	Added (A)	Unbound (B)	Immobilized papain (C)	Activity Yield (%) C/A x 100	Immobilization Yield (%) (A-B)/A x 100
Ist	65197± 2015	2313± 341	50867± 1210	78	96
IInd	69460± 1235	1494± 141	51841± 1339	75	98
IIIrd	69324± 508	2910± 176	49852± 692	72	96
IVth	67636± 2181	3266± 102	50116± 996	74	95

Supported by UNESCO / MIRCEN network

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