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Primers
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Sequence
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5' Arbitrary primers
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P1
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5'-ATTAACCCTCACTAAATGCTGGGGA-3' |
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P2 |
5'-ATTAACCCTCACTAAATCGGTCATAG-3' |
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P3 |
5'-ATTAACCCTCACTAAATGCTGGTGG-3' |
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P4 |
5'-ATTAACCCTCACTAAATGCTGGTAG-3' |
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P5 |
5'-ATTAACCCTCACTAAAGATCTGACTG-3' |
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P6 |
5'-ATTAACCCTCACTAAATGCTGGGTG-3' |
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P7
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5'-ATTAACCCTCACTAAATGCTGTATG-3'
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P8
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5'-ATTAACCCTCACTAAATGGAGCTGG-3'
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P9
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5'-ATTAACCCTCACTAAATGTGGCAGG-3'
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| 3' Anchored oligo-dT primers (30 mer) | |
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T1 |
5'-CATTATGCTGAGTGATATCTTTTTTTTTAA-3' |
| T2 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTAC-3' |
| T3 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTAG-3' |
| T4 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTCA-3' |
| T5 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTCC-3' |
| T6 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTCG-3' |
| T7 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTGA-3' |
| T8 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTGC-3' |
| T9 | 5'-CATTATGCTGAGTGATATCTTTTTTTTTGG-3' |
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