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Indicate down-regulated (suppressed) transcripts following water stress. |
Indicate up-regulated (induced) transcripts following water stress. |
Indicate new (activated) transcripts following water stress. |
| (A) Primer combination P2/T7 was effective to identify down-regulated transcripts. |
| (B) P2/T4. |
| (C) P2/T8 were good in identifying both down- and up-regulated transcripts. |
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Figure 1. DDRT profile of stressed and irrigated peanut plants. Differentially displayed bands in DDRT-PCR of total RNA extracted from water stress (S) and irrigated (I) peanut (Arachis hypogaea L. cv. Florunner) plants using different primer combinations (P indicate arbitrary and T indicate anchored primer, for details see Table 1). Two lanes (a, b) under S and I reflect two different dilutions of cDNA used in DDRT reaction. |
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