Protocol for regeneration in vitro of Arachis hypogaea L.

María Florencia Cucco
Departamento de Microbiología e Inmunología
Universidad Nacional de Río Cuarto
Ruta 8 Km. 601 - 5800 Río Cuarto, Argentina.
Tel: 0358-4676200
E-mail: florcucco@infovia.com.ar

Alberto Daniel Rossi Jaume*
Departamento de Ciencias Naturales
Universidad Nacional de Río Cuarto
Ruta 8 Km. 601 - 5800 Río Cuarto, Argentina.
Tel: 0358-4676200
E-mail: ARossiJaume@exa.unrc.edu.ar

*Corresponding author

Financial Support: Secretary of Science and Technology, Universidad Nacional de Río Cuarto.

Keywords: Growth regulators, Herbicides, Somatic embryogenesis.

The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l^-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l^-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage.

Abbreviations: MS: Murashige and Skoog medium (1962); 2,4-D: 2,4-dichlorophenoxyacetic acid; BAP: Benzylaminopurine; NAA: Naphthaleneacetic acid; Picloram [Picloram acid (4-amino-3, 5, 6 trichloropicolinic acid)], DMA (Dimetilamine salts of 3,6-dichloro-2-methoxybenzoic acid); INTA: Instituto Nacional de Tecnología Agropecuaria.