Region A

a 
Pear
Bean
 
PYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLSWTNLSG
***  *      ** **** *****  *  *    ** **
PYLNFLYIGGINNLVGPIPPAIAKLTQLHYLYITHTNVSG  40
    
 
  
Pear
Bean
 
SVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALR
      ** * *  ** * * * *  * * * *****   
AIPDFLSQIKTLVTLDFSYNALSGTLPPSISSLPNLGGIT  80
     
  
Pear
Bean
 
LDRNKLTGHIPISFGQFIGNVPDLYLSHNQLSGNIPTSFA
 * *   * ** * * *         * * * * **  **
FDGNRISGAIPDSYGSFSKLFTAMTISRNRLTGKIPPTFA  120
     
  
Pear
Bean
 
QMDFTSIDLSRNKLEGDASVIFGLNKTTQIV
       ***** ******* **  *  
NLNLAFVDLSRNMLEGDASVLFGSDKNTKKI           151

 

Region B

b 
IPGIPL
 
5'-C-GAATTC-CC GCC ATC GCT AAA CT -3'

             A   T   T   C   G
  
AA (REGION A)
 
           P    A   I   A   K   L
  
IPGIPR
 
5'-CA-GGATCC-CTC CAG CAT GTT CCG A -3'

             T   G    T      A   T
  
AA (REGION B)
 
          G   E   L   M/K N   R

 

Figure 1. (a) Amino acid sequence alignment of the central protein regions from bean and pear PGIPs (Toubart, et al., 1992; Stotz et al, 1993). Amino acid identities are indicated with an asterisk. The conserved regions A and B, which were used to design degenerate primers, are indicated in bold. (b) Degenerate primers, IPGIPL and IPGIPR and their corresponding amino acid (AA) sequences.

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