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Table 1. Time constants and open probabilities of mSlo and mSlo-GFP channels
|
Channel type |
t c1 (msec) |
t c2 (msec) |
t c3 (msec) |
t o (msec) |
Po (%) |
n |
|
mSlo |
0.453 ± 0.103 |
4.30 ± 0.54 |
19.1 ± 2.9 |
5.26 ± 0.92 |
64.2 ± 2.1 |
4 |
|
mSlo-GFP |
0.544 ± 0.101 |
4.43 ± 0.96 |
16.5 ± 4.4 |
6.07 ± 1.27 |
65.2 ± 2.0 |
4 |
Analysis of dwell time histograms (representative histograms are shown in figures 4 and 5) measured at + 30 mV in symmetrical 150 mM KCl and 100 m M Ca2+. Closed time histograms were fitted with three exponentials (t c1 - t c3), and open time histograms were fitted with one exponential (t o). Each value represents the mean ± SE of the number of measurements reported (n), each representing control recordings of a single channel in a separate bilayer.