Table 1. Time constants and open probabilities of mSlo and mSlo-GFP channels

 

Channel type

t c1 (msec)

t c2 (msec)

t c3 (msec)

t o (msec)

Po (%)

n

mSlo

0.453 ± 0.103

4.30 ± 0.54

19.1 ± 2.9

5.26 ± 0.92

64.2 ± 2.1

4

mSlo-GFP

0.544 ± 0.101

4.43 ± 0.96

16.5 ± 4.4

6.07 ± 1.27

65.2 ± 2.0

4

 

Analysis of dwell time histograms (representative histograms are shown in figures 4 and 5) measured at + 30 mV in symmetrical 150 mM KCl and 100 m M Ca2+. Closed time histograms were fitted with three exponentials (t c1 - t c3), and open time histograms were fitted with one exponential (t o). Each value represents the mean ± SE of the number of measurements reported (n), each representing control recordings of a single channel in a separate bilayer.

Supported by UNESCO / MIRCEN network
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