Figure 3. Currents through mSlo and mSlo-GFP channels from transfected COS-7 cell membranes reconstituted into lipid bilyers.

A: The current-voltage relationship of mSlo channels, with the solid line representing a linear fit of 8 single channel experiments (n=5 for each data point). The standard error of the mean (SEM) is less than the width of the points and the average channel conductance was 265 pS. B: Voltage dependence of a typical single mSlo channel. The solid line represents a least squares fit of the Boltzman distribution. C: Single channel current (mSlo) activation by free Calcium added to the internal side of the channel in 150 mM symmetrical KCl. The data was least-squares fitted with the Hill equation (KD = 13.9 m M, n = 2.9, Vmax = 94). D: The current-voltage relationship of mSlo-GFP channels, with the solid line representing a linear fit of 9 single channel experiments (n=5 for each data point, except for +60 mV, where n =1). The standard error of the mean (SEM) is less than the width of the points and the average channel conductance was 272 pS. E: Voltage dependence of a single mSlo-GFP channel in 150 mM symmetrical KCl. The solid line represents a least squares fit of the Boltzman distribution. F: Single channel current (mSlo-GFP) activation by free Calcium added to the internal side of the channel in 150 mM symmetrical KCl. The data was least-squares fitted with the Hill equation (KD = 11.2 m M, n = 2.5, Vmax = 84).

Supported by UNESCO / MIRCEN network
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