Figure 5. Follow-up maintenance experiments of cells transfected with the two alternative vectors pCMVL (Panel A) and p103 (Panel B).
Figure 5. Follow-up maintenance experiments of cells transfected with the two alternative vectors pCMVL (Panel A) and p103 (Panel B).Parallel transfection experiments were carried out with a ratio of Transfectam to DNA of 1.0 for both plasmids. 5.0 m g of total unrestricted DNA and 10.0 m g of restricted DNA were loaded into each gel slot. Restriction sites for either plasmid are: One ScaI site (linearises cccDNA). Three EcoRI sites (3 fragments). (NT) Non Transfected control cell DNA; Control lanes (pCMVL or p103, 109 copies each or 1.0 ng); (GO) 30 min postransfection DNA; (G1) 96 h postransfection cell DNA, (G2) Second passage cell DNA ; (G3) Third passage cell DNA; (G4) Fourth passage cell DNA; (G5) Fifth passage cell DNA; (G0-5) Non passage cell DNA. For each passage, lane numbers mean as follows: 1.- unrestricted DNA; 2.- DNA restricted with ScaI; 3.- DNA restricted with EcoRI.
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