Figure 3. Comparison of DEAE Dextran with Transfectam Reagent as DNA carriers for transfection.
Figure 3. Comparison of DEAE Dextran with Transfectam Reagent as DNA carriers for transfection.Duplicate independent transfections were carried out using pCMVL DNA (5.0 m g) in the presence of DEAE Dextran (1.0 m g) or Transfectam (12.5 m g). Total DNA was extracted and submitted to dot blot hybridisation at two different amounts (2.0 and 1.0 m g, respectively), using linearised DIG-labelled pCMVL as a probe. Control Lane at left, duplicates of pCMVL DNA (0.6 m g), and at right, duplicates of total salmon sperm DNA (1.0 m g).
Home | Mail to Editor | Search | Archive