Table 1. Comparison of probiotic properties between Lactobacillus plantarum DW3 and the commercial probiotic Lactobacillus casei R.



Probiotic properties

DW3

R

 Growth at 0.15% bile salt

+

+

 Growth at 0.30% bile salt

+

+

 Growth at pH 2

-

-

 Growth at pH 3, 4, 5

+

+

 Growth at pH 8

+

-

 Growth at pH 9

-

-

 Oxygen effect on growth

No

No

 Hydrophobicity (% ± SD)

10.77 ± 0.05

11.79 ± 0.23

 Utilization of protein

+

+

 Utilization of starch

+

-

 Utilization of lipid

-

-

 Vitamin B12 requirement

-

-

 Growth in SPY21 and MRS

+ (r > 0.8, p < 0.05)

+ (r > 0.8, p < 0.05)

 Doubling time (min) in SPY2

59

71

 Antibiotic susceptibility test2

Resistance to VC, BC,
GT, KC, ST, NF, PB

Resistance to VC, BC,
GT, KC, ST, NF, PB

No = No significant difference (p > 0.01) between growth with or without oxygen present; SD = standard deviation of three determinations.
1Animal free medium.
2Antibiotics used: VC: vancomycin (30 µg), BC: bacitracin (10 µg), GT: gentamycin (10 µg), KC: kanamycin (30 µg), ST: streptomycin (10 µg), NF: norfloxacin (10 µg), PB: polymyxin B (300 µg), ampicillin (10 µg), cephalothin (30 µg), ceftazidime (30 µg), chloramphenical (30 µg), erythromycin (15 µg), penicillin G (10 U), ceferperazone (75 µg), tetracycline (30 µg).


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