Figure 2. HPLC profile of the cell free supernatant of Lactobacillus plantarum DW3.
Column: Hypersil ODS column C18 RP (250 x 4.0 mm, HP, particle size 5 µm)
Elution: linear gradient from 25% acetronitrile in 75% water (at 0-3.0 min, flow rate 1 ml/min) to 50% acetronitrile in 50% water (in 4-6 min, flow rate 1 ml/min) and acetronitrile was adjusted to 100% (in 8-12 min, flow rate 1 ml/min).
Detection: diode array detector set at 210 nm.