Figure 1. BAA1 was expressed in the binary plant expression vector pIG 121 Hm. Nos-p: nos promoter; HPT: gene encoding hygromycin phosphotransferase; Nos-t: nos terminator; 35S-p: CMV 35S promoter; BAA1: gene encoding fruit bromelain; LB: left border; and RB: right border. Primers for RT-PCR for cloning of BAA1 gene (arrows) and primers for real-time PCR of BAA1 gene (arrowhead) are indicated.