Table 4. Degradation of (-)-alpha-pinene (1,0 ml of a methanolic solution of 50 mg/ml) in reaction medium (50 ml). Similar profiles were obtained with 50 mL of the following types of solvents evaluated separately: distilled water, Milli-Q® water, phosphate buffer, MS medium prepared with Milli-Q® water, or MS medium prepared with distilled water. Numbers represent mean percentages ± standard deviation (four replications) of components starting with 100% alpha-pinene at the experimental onset.

3 hrs

Day 1

Day 3

Day 5

Day 7

Day 10

Day 15

 (-)-alpha-pinene

2.2 ± 1.3

0.0

0.0

0.0

0.0

0.0

0.0

 campholenal

7.2 ± 2.5

3.2 ± 0.9

2.0 ± 0.9

1.5 ± 0.6

0.8 ± 0.2

1.6 ± 0.4

0.2 ± 0.1

 trans-pinocarveol

10.9 ± 3.7

6.5 ± 2.7

5.6 ± 2.3

4.4 ± 0.8

3.1 ± 1.7

3.0 ± 1.4

1.0 ± 0.3

 cis-verbenol

2.1 ± 0.5

2.3 ± 1.3

3.1 ± 0.8

2.7 ± 1.0

2.0 ± 1.4

2.9 ± 0.4

2.7 ± 0.1

 trans-verbenol

62.5 ± 3.5

64.6 ± 6.0

67.5 ± 2.9

67.6 ± 2.4

64.6 ± 2.4

59.4 ± 1.6

60.0 ± 2.5

 myrtenol

6.7 ± 2.5

6.1 ± 1.2

6.6 ± 0.5

6.5 ± 0.6

6.0 ± 0.5

6.5 ± 0.5

6.9 ± 0.4

 (-)-verbenone

7.6 ± 0.8

8.1 ± 1.3

10.9 ± 0.8

12.9 ± 0.7

17.6 ± 2.2

19.6 ± 1.3

23.8 ± 0.3

 myrtanol

2.3 ± 1.5

1.7 ± 0.9

2.1 ± 0.3

2.2 ± 0.4

2.7 ± 0.6

2.6 ± 0.2

2.7 ± 0.3