Table 1. Characteristics of the microcarriers and conditions in which they were tested in spinner-flask cultures of the CHOZMD cell.

Microcarrier

Outstanding features1

Particle size (µm)
and shape1

Densities
(based on medium volume)

Microcarrier
(g.L-1)

Cells
(cell.mL-1)

 Cytodex 1

Non-porous, dextran matrix
positively charged, hydrophilic

Bead (190)

3.5

1.0 x 105

 Cultispher S

Porous, gelatin matrix2
uncharged, hydrophilic

Bead
(125 - 212)

2.0

1.0 x 105

 Pronectin F

Non porous, coated with pronectin3
uncharged, hydrophilic

Bead
(130 - 380)

22.0

1.1 x 105

 Immobasil FS

Porous, silicone rubber matrix
negatively charged4, hydrophobic4
highlypermeable to oxygen

Disc
(800 x 250)

26.0

1.0 x 105

1Information supplied by manufacturers.
2Polypeptide extracted from animal tissue where it functions as a promoter of cell adhesion.
3Recombinant polypeptide with multiple copies of the sequence RGD (Arg-Gly-Asp), a common motif recognized by specific cell-surface receptors during adhesion.
4Data from Nakari-Setälä et al. (2002).

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