Table 2. Effect of fermentation technologies on the population of Lactobacillus rhamnosus RW-9595M, medium CRV and glucose/lactate levels in the fermented media.


Fermentation
technology

Population
(CFU/ml or /g)

CRV

Glucose
(g/l)

Lactate
(g/l)

Free cell control

3.0 (0.9) x 109

5.07 (0.32)

0

37.3 (3.2)

Immobilized cells

 

 

 

 

Beads

3.0 (1.5) x 1010

4.41 (0.92)

0.4 (0.03)

38.7 (2.6)

Free cells outside beads

1.1 (2.4) x 109

 

 

 

Fed batch

 

 

 

 

 Beginning

8.3 (2.0) x 108

1.26 (0.17)

0

9.7 (1.7)

 1 hr

8.0 (1.9) x 108

1.52 (0.18)

0.9 (0.1)

16.0 (1.8)

 2 hrs

2.0 (0.9) x 109

1.60 (0.30)

2.5 (0.6)

21.6 (3.0)

 3 hrs

2.1 (0.2) x 109

2.23 (0.13)

2.5 (0.3)

22.5 (1.3)

 4 hrs

2.6 (0.4) x 109

2.50 (0.42)

5.6 (0.4)

33.4 (4.2)

 5 hrs

3.9 (1.4) x 109

3.25 (0.29)

7.9 (0.5)

38.7 (2.9)

Values in brackets represent SEM obtained from three independent assays.


Supported by UNESCO / MIRCEN network