Figure 3. Chromatographic profile of small- and manufacturing columns for immunoaffinity chromatography (a-b) and positive anion-exchange chromatography (c-d). Biological materials applied in both cases were from the same manufacturing batch with a charge equivalent to 1 mg/mL resin for immunoaffinity and anion-exchange chromatography respectively. Reg. Regeneration step.
(a) For immunoaffinity chromatography the small column is 1.6 cm diameter by 10 cm height.
(b) For immunoaffinity chromatography the manufacturing column is 20 cm diameter by 38 cm height.
(c) For positive anion-exchange chromatography the small column is 2.6 cm diameter by 5 cm height.
(d) For positive anion-exchange chromatography the manufacturing column is 30 cm diameter by 30 cm height.