Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 1, Issue of January 15, 2007
© 2007 by Pontificia Universidad Católica de Valparaíso -- Chile Received May 19, 2006 / Accepted August 21, 2006
DOI: 10.2225/vol10-issue1-fulltext-9  

Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal

Klaus-Thomas Haensch
Institute of Vegetable and Ornamental Crops Großbeeren/Erfurt e.V.
Department Plant Propagation
Kühnhäuser Str. 101, D-99189 Erfurt-Kühnhausen
Tel: 49 0 36 201 785 224
Fax: 49 0 36 201 785 250


Financial support: This investigation was undertaken with the support of the Ministries of Agriculture of the Federal Republic of Germany and the States of Brandenburg and Thüringen.

Keywords: auxin, cytokinin, pelargonium, propagation, somatic embryogenesis.


2,4 D: 2,4 dichlorophenoxyacetic acid
BAP: 6 benzylaminopurine

Full Text

This long-term study demonstrates for the first time that it is possible to propagate embryogenic cultures in pelargoniums and to subsequently initiate the differentiation of embryos using the cultivar Madame Layal (Pelargonium x domesticum). Propagation of callus was only possible with combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP), which gave rise to embryos from the primary culture stage on. However, the propagation of cells, as well as the differentiation of embryos, was inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in pelargoniums are discussed.

Supported by UNESCO / MIRCEN network