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Characterization of a gelatin/chitosan/hyaluronan scaffold-polymer Javier
Enrione Fernando
Osorio Daniel
López Caroline
Weinstein-Oppenheimer Miguel
A. Fuentes Ricardo
Ceriani Donald
I. Brown Fernando
Albornoz Elizabeth
Sánchez Patricio
Villalobos Rodrigo
A. Somoza Manuel
E. Young Cristian
A. Acevedo* *Corresponding author Financial support: The authors wish to thank CONICYT by: FONDEF-Grant D07I1075, FONDECYT-Grant 11075053, FONDECYT-Grant 11090051, PBCT-Grant PSD62 and Doctoral Fellowships for R.A. Somoza. Keywords: chitosan, fibrin, gelatine, hyaluronic acid, scaffold.
Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a gelatin/chitosan/hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures up to 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin, reporting biological behaviour and its mechanical properties. |