Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 5, Issue of October 15, 2006
 

Table 3. Coded levels and real values (in parentheses) for the third factorial design (17 trials) and glucosyltransferase activity (U/mL).


Assay

Variable (g/L)

Enzyme activity

(U/mL)

SCMa

BPb

YEPc

1

-1 (130.24)

-1 (11.07)

-1 (9.05)

4.23

2

+1 (189.76)

-1 (11.07)

-1 (9.05)

3.97

3

-1 (130.24)

+1(28.93)

-1 (9.05)

5.07

4

+1 (189.76)

+1 (28.93)

-1 (9.05)

3.87

5

-1 (130.24)

-1 (11.07)

+1 (20.95)

4.85

6

+1 (189.76)

-1 (11.07)

+1 (20.95)

4.23

7

-1 (130.24)

+1(28.93)

+1 (20.95)

5.53

8

+1 (189.76)

+1 (28.93)

+1 (20.95)

3.15

9

-1.68 (110)

0 (20)

0 (15)

4.89

10

+1.68 (210)

0 (20)

0 (15)

3.27

11

0 (160)

-1.68 (5)

0 (15)

6.14

12

0 (160)

+1.68 (35)

0 (15)

4.32

13

0 (160)

0 (20)

-1.68 (5)

5.19

14

0 (160)

0 (20)

+1.68 (25)

4.91

15

0 (160)

0 (20)

0 (15)

7.47

16

0 (160)

0 (20)

0 (15)

7.12

17

0 (160)

0 (20)

0 (15)

7.19

asugar cane molasses; bbacteriological peptone; cyeast extract Prodex Lac SD®.

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