Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 5, Issue of October 15, 2006
 

Table 2. Coded levels and real values (in parentheses) for the second factorial design (17 trials) and glucosyltransferase activity (U/mL).


Assay

Variable (g/L)

Enzyme activity

(U/mL)

SCMa

BPb

YEPc

1

-1 (120.24)

-1 (11.07)

-1 (18.10)

6.34

2

+1 (179.76)

-1 (11.07)

-1 (18.10)

8.28

3

-1 (120.24)

+1(28.93)

-1 (18.10)

7.56

4

+1 (179.76)

+1 (28.93)

-1 (18.10)

8.97

5

-1 (120.24)

-1 (11.07)

+1 (41.90)

7.39

6

+1 (179.76)

-1 (11.07)

+1 (41.90)

8.68

7

-1 (120.24)

+1(28.93)

+1 (41.90)

6.63

8

+1 (179.76)

+1 (28.93)

+1 (41.90)

6.28

9

-1.68 (100)

0 (20)

0 (30)

4.99

10

+1.68 (200)

0 (20)

0 (30)

7.01

11

0 (150)

-1.68 (5)

0 (30)

7.06

12

0 (150)

+1.68 (35)

0 (30)

7.47

13

0 (150)

0 (20)

-1.68 (10)

8.47

14

0 (150)

0 (20)

+1.68 (50)

7.06

15

0 (150)

0 (20)

0 (30)

8.43

16

0 (150)

0 (20)

0 (30)

8.23

17

0 (150)

0 (20)

0 (30)

7.83

asugar cane molasses; bbacteriological peptone; cyeast extract Prodex Lac SD®.

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