Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 4, Issue of July 15, 2006
 


Figure 4. Identification of LPs containing XlMyo7a λgt10 cDNA clones. LPs were prepared as described in Figure 1. Using 3 µl as template in a 20 µl PCR reaction, 40 LPs were screened by PCR using the isoform specific primers for Xlmyo7a. The products were electrophoresed on a 3% agarose gel. The 88 bp product was detected in LPs 7 and 24. On both the top and bottom tiers of the gel, Lambda/HindIII markers were run in lane 1) The following templates were used in the PCR reactions whose products are run in lanes 2-4; lane 2) pXlmyo7a, a plasmid containing the fragment from which the isoform specific primers used to screen the library were derived; lane 3) no DNA template; lane 4) λPS103, a plate lysate of a purified lambda clone of the XlMyo1d myosin isoform.


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