Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 4, Issue of July 15, 2006
 


Figure 3. SP screens for two myosin isoforms. SPs from step 3 of Figure 1 were screened in two separate sets of PCR reactions to identify SPs containing 7a cDNA clones (top lanes) or 1d (bottom lanes). Using 1 µl as a template per PCR, 17 of the 20 SPs were screened. Lanes designated M contain the same markers as in Figure 2 loaded at a lower concentration. The smallest visible band corresponds to the 506, 517 bp doublet. The 7a screen identified two positive SPs, numbers 5 and 10, and the 1d screen identified four SPs, numbers 4, 6, 12, and 17. Lanes designated (+) contain PCR reaction product in which the cloned DNA fragments from which the primer pairs were derived were used as template. Lanes (-) contain products of PCR reactions with no added DNA template.


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