Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 4, Issue of July 15, 2006
 


Figure 1. The LP screening method. The strategy is based on PCR detection of λ phage cDNA clones in pools created from high and low density plates. The integrity of low density plates in step 7 is preserved because phage are not eluted directly from the agar, neither by flooding the plate, nor by elution of phage from slices of agar from the plate. Instead plaques adhering to a nitrocellulose filter placed on the plate are washed off and used as template for the PCR screen. Once a positive low density plate is identified, individual phage eluted from agar plugs can be screened to identify the clone. Details of the protocol are described in the Materials and Methods.


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