Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 4, Issue of July 15, 2006
 

Table 1. Summary of treatments subjected to leaf segments of Nicotiana tabacum prior to co-cultivation with A. rhizogenes.

Treatment

Sequence of treatments prior to co-cultivation or during co-cultivation

A

Manual wounding

B

Ultra-sonication

C1

Ultra-sonication + Pectinasea 0.1%

C2

Ultra-sonication + Pectinasea 0.5%

C3

Ultra-sonication + Pectinasea 1%

D1

Ultra-sonication + Cellulasea 0.1%

D2

Ultra-sonication + Cellulasea 0.5%

D3

Ultra-sonication + Cellulasea 1%

E1

Ultra-sonication + Pectinase and Cellulasea 0.1%

E2

Ultra-sonication + Pectinase and Cellulasea 0.5%

E3

Ultra-sonication + Pectinase and Cellulasea 1%

F1

Ultra-sonication + acetosyringoneb 50 ÁM

F2

Ultra-sonication + acetosyringoneb 100 ÁM

F3

Ultra-sonication + acetosyringoneb 150 ÁM

G1

Ultra-sonication + CaCl2b 5 mM

G2

Ultra-sonication + CaCl2b 10 mM

G3

Ultra-sonication + CaCl2b 20 mM

a Prior to co-cultivation with A. rhizogenes.
b During co-cultivation with A. rhizogenes.

 

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