Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 3, Issue of April 15, 2006
 


Table 1. Primers used in PCR.

Primer

5´ to 3´ Sequence

Target

Ta

Product

Method

IVR1
IVR2

5´-CCGCTGTATCACAAGGGCTGGTACC-3´
5´-GGAGCCCGTGTAGAGCATGACGATC-3´

Maize Invertase

68ēC

226 bp

Specie specific

LE1
LE2

5´-AAGCAACCAAACATGATCCTC-3´
5´-ATGGATCTGATAGAATTGACGTTA-3´

Soy lectin

44ēC

407 bp

Specie specific

35S-1
35S-2

5´-GCTCCTACAAATGCCATCA-3´
5´-GATAGTGGGATTGTGCGTCA-3´

CaMV 35S

55ēC

195 bp

Screening

GM07
GM08

5´-ATCCCACTATCCTTCGCAAGA-3´
5´-TGGGGTTTATGGAAATTGGAA-3´

EPSPS

57ēC

169 bp

Transgene specific

CRY1
CRY2

5´-ACCATCAACAGCCGCTACAACGACC-3´
5´-TGGGGAACAGGCTCACGATGTCCAG-3´

CryIA(b)

71ēC

184 bp

Transgene specific


Supported by UNESCO / MIRCEN network