Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 2, Issue of April 15, 2006
 


Table 2. Determination of PAb-Mn SOD in different tissues of pearl millet seedlings. Two-day-old resistant (IP 18293) and susceptible (23B) pearl millet genotypes were inoculated with S. graminicola zoospores (4 x 104 zoospores/ml). Root and shoot portions of seedlings were excised 4 hrs of post inoculation and used for the enzyme extraction. 50 g of each sample was loaded to the wells of ELISA plate. Primary antibody was used at a dilution of 1:20,000. The reaction was read at 410 nm using microtitre plate reader. Data are mean (SD) of three independent experiments with reading determined in triplicates.

Part of the seedling

Absorbance at 410 nm

Resistant

Susceptible

Uninoculated

Inoculated

Uninoculated

Inoculated

Root

0.31 0.031

0.85 0.025

0.37 0.018

0.52 0.024

Shoot

0.46 0.034

0.25 0.018

0.59 0.037

0.57 0.017


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