Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 2, Issue of April 15, 2006
 


Table 1. Determination of PAb-Mn SOD in resistant and susceptible pearl millet genotype after inoculation with S. graminicola. Two-day-old resistant (IP 18293) and susceptible (23B) pearl millet seedlings were inoculated with zoospore suspension of S. graminicola (40 x 104 zoospores/ml). Seedlings harvested at regular intervals of two hours up to 24 hrs were used for the enzyme extraction. Protein (50 μg) were loaded to the wells of ELISA plate. Primary antibody was used at a dilution of 1:20,000. The reaction was read at 410 nm using microtitre plate reader. Data are mean ( SD) of three independent experiments with reading determined in triplicates.

Time after inoculation

Absorbance at 410 nm

Resistant

Susceptible

Uninoculated

Inoculated

Uninoculated

Inoculated

0

0.22 0.017

0.22 0.019

0.24 0.0204

0.23 0.016

2

0.26 0.018

0.27 0.018

0.29 0.0168

0.24 0.024

4

0.28 0.041

0.51 0.018

0.38 0.0246

0.30 0.018

6

0.33 0.031

0.40 0.016

0.32 0.019

0.29 0.031

8

0.30 0.037

0.37 0.041

0.28 0.014

0.27 0.017

10

0.27 0.034

0.35 0.032

0.24 0.0179

0.23 0.018

12

0.24 0.024

0.31 0.029

0.22 0.0181

0.23 0.031

14

0.24 0.025

0.27 0.028

0.23 0.0163

0.23 0.018

16

0.24 0.035

0.27 0.021

0.23 0.028

0.24 0.02

18

0.23 0.031

0.27 0.023

0.23 0.021

0.25 0.017

20

0.23 0.016

0.26 0.026

0.23 0.024

0.25 0.018

22

0.22 0.021

0.25 0.015

0.23 0.013

0.24 0.016

24

0.22 0.025

0.24 0.016

0.23 0.015

0.24 0.041


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