Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 2, Issue of April 15, 2006



Figure 4. Immunoblotting for the detection of the 35 kDa protein. Two-day-old resistant (IP 18293) and susceptible (23B) pearl millet seedlings were inoculated with zoospore suspension of S. graminicola (40 x 104 zoospores/ml). Seedlings harvested at 4 hrs post-inoculation were used for the enzyme extraction. Primary antibody was used at a dilution of 1:5000. SC: Susceptible control, SI: Susceptible incoulated, RC: Resistant control, RI: Resistant Inoculated. Band intensity was quantitated by bioprofile image analysis (Vilber Lourmat, France).

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