Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2006 by Universidad Católica de Valparaíso -- Chile
Vol. 9 No. 2, Issue of April 15, 2006
 

 

 

Figure 3. Immnodetection of the antibody specificity by Dot-immunobinding assay (DIBA) for:

(a) crude and pure antigen.
(b) seedlings extracts of different pearl millet genotypes
(c) root and shoot of resistant and susceptible pearl millet genotypes.

Two-day-old resistant and susceptible pearl millet seedlings were inoculated with zoospore suspension of S. graminicola (4 x 104 zoospores/ml). Primary antibody was used at a dilution of 1:5000. (w, distilled water treatment; c, crude SOD extract; p, pure Mn-SOD, (s, susceptible; r, resistant; UI, uninoculated; and I, inoculated)


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