Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 2, Issue of August 15, 2005
 

 


Figure 5. Western-blot of hairy root biomass. The lines were loaded with equal volumes of samples. Line 1: Standard IgG Antibody; line 2: N. tabacum wild type; line 3: N. tabacum wild type + 5% DMSO; line 4: Ac2 + 2.8% DMSO; line 5: Ac2 + 5% DMSO; line 6: Ac2 control; line 7: AcK6 + 2.8% DMSO; line 8: AcK6+5%; line 9: AcK6 control. Coating: gaot anti gamma mouse chain conjugated with peroxidase (1:1000) in 1% (P/V) non fat milk. Antibody binding was detected after incubation with Supersignal West Pico Chemiluminiscent Substrate (Pierce Chem.) after 25 min of exposure.

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