Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005
 


Table 1. Transformation efficiency of plasmid DNA to different E. coli strains (Average).

 
XL1 blue E. coli strains
DH5a E. coli strains
TG1 E. coli strains
plasmid
Normal method (Sambrook, 1989)
Quick method(Chen, 2001)
This improved method
Normal method (Sambrook 1989)
Quick method(Chen, 2001)
This improved method
Normal method (Sambrook 1989)
Quick method(Chen, 2001)
This improved method

pUC18

6.51x106

7.1x105

8.18x108

2.31x106

3.5x105

4.15x108

2.01x106

3.8x105

4.5x108

pUGFP

7.79x106

6.7 x105

8.85x108

1.66x106

3.2 x105

2.63x108

1.25x106

2.2 x105

4.72x108

pAHC25

6.86x106

7.8 x105

9.09x108

1.56x106

2.7 x105

4.13x108

1.46x106

2.9 x105

5.39x108

p1Ax1

7.13x106

6.4 x105

8.6 x108

1.25x106

2.3x105

4.52x108

1.08x106

2.7 x105

4.26x108

p1Dx5

7.09x106

6.98x105

8.9 x108

1.31x106

2.08x105

3.99x108

1.21x106

2.08x105

4.19x108

p1Dy10

8.17x106

6.75x105

7.8x108

1.20x106

1.97x105

3.56x108

1.31x106

1.97x105

3.25x108


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