Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005


Fig 7. Schematic representation of inverse PCR. This involves restriction digestion of genomic DNA from mutant plant with an appropriate enzyme that cuts (preferably cuts once within the T-DNA) followed by self-ligation. The circularized ligation products are used for PCR amplification using appropriate primers from the T-DNA region. The flanking plant DNA is represented by green line. The appropriate primers (forward and reverse) are indicated by blue and red arrows.

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