Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005


Figure 6. Schematic representation of plasmid rescue. This strategy involves restriction digestion ofgenomic DNA from mutant plant with an appropriate enzyme (preferably with an enzyme which does not cut within the T-DNA). Self ligated molecules are used for transformation of E. coli. The self-ligated fragments containing the T-DNA along with the flanking sequences are able to survive and are rescued as plasmid because of the presence of origin of replication (Ori), shown here in blue. The plant DNA is indicated by green line. The restriction sites are shown as red arrows.

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