Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005


Figure 5. Schematic representation of TAIL PCR. This strategy involves three consecutive rounds of PCR, performed with a set of three nested T-DNA specific primers (SP1, SP2 and SP3) and a small, arbitrary primer. The positions of three nested primers in the T-DNA region are indicated by blue, red and green arrows respectively. AD is arbitrary degenerate primer indicated by purple arrow. T-DNA flanking region is indicated by green line.

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