Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005
 


Table 1. Primers used in this study.

Primers

Sequences

Applications

P1 5-CCCAAGCTTGAATGCTTGTATTGACC
To clone the upstream sequence
P2 5-GCGTCGACGGATTTATCTCCTCTTG
P3 5-ATGGTGAGCAAGGGCGAG
To delete the additional sequence
P4 5-GGATTTATCTCCTACTTG
P5 5-CCTGCGTTATCCCCTGATTCTGTGG
To select the deletion mutants
P6 5-GAACAGCTCCTCGCCCTTGCTCACC
P7 5-CATGCAGACTTACAACAACCC
To clone the isiAB gene fragment
P8 5-AGCCAACTGTTTGACTACC
PdL 5-TCAATACAAGCATTCAAG
Paired with the below primers to produce a series of 5' end deletion mutations of the upstream sequence of cpcB gene

 

PdR1 5-AGAAGGAAGGCGAATGTTG
PdR2 5-ATTCTTCTCATAAACCCTG
PdR3 5-AGCCTTTGTCGATGGTTCG
PdR4 5-ACCGATGGATTGATTGTCG

PdR5

5-CCCAACTCAACTCTAAGC


Note: HindIII site (AAGCTT) and SalI site (GTCGAC) are blackened.


Supported by UNESCO / MIRCEN network