Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005
 


Table 1. Media composition for oil palm tissue culture and genetic transformation studies.

Media Code

N60

N62.5

N6FET

R68

Basic Media

N6a

N6a

N6a

B5b

Macronutrients (mg/l)

CaCl22H2O

166

166

166

-

CaCl

-

-

-

113.24

KH2PO4

400

400

400

-

NaH2PO4

-

-

-

130.5

MgSO47H2O

185

185

185

-

MgSO4

-

-

-

112.09

KNO3

2830

2830

2830

2500

(NH4)2SO4

463

463

463

134

Micronutrients (mg/l)

KI

0.8

0.8

0.8

0.75

H3BO3

1.6

1.6

1.6

3

MnSO44H2O

4.4

4.4

4.4

10

ZnSO47H2O

1.5

1.5

1.5

2

CoCl26H2O

-

-

-

0.025

CuSO45H2O

-

-

-

0.025

Na2MoO42H2O

-

-

-

0.25

FeSO47H2O

27.85

27.85

27.85

27.85

Na2EDTA

37.25

37.25

37.25

37.25

Vitamins and sugars (mg/l)

Myo-inositol

100

100

110

100

Nicotinic acid

0.5

0.5

500

1

Pyridoxine-HCl

0.5

0.5

-

1

Thiamine-HCl

1.0

1.0

-

10

Ascorbic acid

-

-

250

-

Sucrose

30000

30000

30000

30000

Kao's vitaminsc

-

-

1X

-

Y3 vitaminsd

-

-

1X

-

Amino acids, hormones and antibiotics (mg/l)

2,4-D

-

2.5

110

0.1

Cysteine

-

-

500

-

Others (mg/l)

Gelrite

2200

2200

2200

2200

PVP-40

-

-

5000

-

Activated Charcoal

-

-

3000

3000

 

pH

5.8

5.8

5.8

5.8

a. Chu et al. 1975;
b. Gamborg et al. 1976;
c. Kao's vitamins: 100 mg/l myo-inositol, 1 mg/l nicotinic acid, 1 mg/l pyridoxine HCl, 1mg/l Thiamine HCl, 1mg/l D-calcium panthothenate, 0.4 mg/l folic acid, 0.02 mg/l p-aminobenzoic acid, 0.01 mg/l biotin, 1 mg/l choline chloride, 0.2 mg/l riboflavin, 2 mg/l ascorbic acid, 0.01mg/l vitamin A, 0.01 mg/l vitamin D3 and 0.02 mg/l vitamin B12;
d. Y3 vitamins: 100 mg/l L-glutamine, 120 mg/l L-arginine, 88 mg/l L-asparagine, 100 mg/l myo-inositol, 1 mg/l nicotinic acid, 1 mg/l pyridoxine HCl, 1 mg/l Thiamine HCl;

 

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