Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2005 by Universidad Católica de Valparaíso -- Chile
Vol. 8 No. 1, Issue of April 15, 2005


Figure 5. Histochemical GUS assay on bombarded and co-cultivated IEs and their respective IE-derived tissues.
a) freshly bombarded Ies;
b) halved-IE 3 days after bombardment showing distinct GUS expression throughout the entire IE with highest expression localised at the meristematic region and areas with actively dividing cells;
c) germinating IEs with distinct shoot (sh) and haustarium (h);
d) freshly co-cultivated Ies;
e) halved-IE 7 days after co-cultivation showing distinct GUS expression localised at meristematic region and areas with actively dividing cells;
f) callusing IE 2 weeks after co-cultivation;
g-h) callusing assay, with callus developing on IE-derived tissues expressing gus activity;
i) callus developing from roots of plantlets undergoing callusing assay also showing gus activity;
j) leaf tissues from IE-derived plantlets showing gus activity. Blue coloration of tissues indicates the expression of GUS gene substantiating successful gene transfer.

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