5. Northern blot analysis.
(A) Ten micrograms of total RNA from seeds (white to black pod maturity category) was separated on a denaturing agarose gel, blotted onto nitrocellulose membrane, and hybridized with Gly-1 labeled probe.
(B) Gly-1 expression in developing seeds, a band of approximately 1.6 kb size represent Gly-1 transcript. The smaller band on the blot is the truncated version of Gly-1 transcript.