Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2002 by Universidad Católica de Valparaíso -- Chile
Vol. 5 No. 3, Issue of December 15, 2002
 


Figure 3. Long-term rhIL-2 release by encapsulated genetically engineered CHO cells. Cells microencapsulated with alginate (Δ) or APA/citrate () as described in Figure 2, were cultured in T25 flasks containing 2 ml of compacted beads in 10 ml of medium that was renewed every 48 h. Medium culture samples were taken and assayed for rhIL-2 as described in Materials and Methods. The rhIL-2 production was expressed as means standard error of the mean of 4 independent experiments measured in duplicate. Results were expressed as mean (s.e.m.). Differences between groups were determined by analysis of variance (ANOVA).


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