Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2002 by Universidad Católica de Valparaíso -- Chile
Vol. 5 No. 2, Issue of August 15, 2002
 


Table 3.

Tfd enzyme activities and rates of 3-CB dechlorination in R. eutropha, P. putida and A. lwoffii strains.


Strain/clone

Inducer

CC-1,2- DO (U/mg)

CMCI
(U/mg)

3-CB dechlorination rate (nmols/min/mg)

R.eutropha JMP134

3-CB

0.51

0.08

37

R.eutropha JMP134

-

0.04

0.00

19

R.eutropha JMP222

3-CB

0.00

0.00

0

R. e. JMP222::ITFD5

-

0.03

0.00

2

R. e. JMP222::ITFD5

IPTG

0.40

0.03

31

R. e. JMP222::TFD5

-

0.03

0.00

1

R. e. JMP222::TFD5

3-CB

0.02

0.00

1

R. e. JMP222::DTFD4

-

0.03

ND

1

R. e. JMP222::DTFD4

3-CB

0.32

ND

18

P. putida KT2442

3-CB

0.00

0.00

0

P. p. KT2442::ITFD1

-

0.02

0.00

0

P. p. KT2442::ITFD1

IPTG

0.22

0.02

23

P. p. KT2442::TFD7

-

0.13

0.01

20

P. p. KT2442::TFD7

3-CB

0.08

0.01

12

A. lwoffii RB2

-

0.00

0.00

0.4

A. l. RB2::ITFD3

-

0.00

0.00

1

A. l. RB2::ITFD3

IPTG

0.01

0.00

2


Values are averages from two independent measurements. Deviations were less than 10%, and 15%, for determinations of enzyme activities, and dechlorination rates, respectively. Growth substrate concentration was always 3 mM benzoate. CC-1,2-DO: Chlorocatechol-1,2-dioxygenase. CMCI: Chloromuconate cycloisomerase. 3-CB: 3-chlorobenzoate. IPTG: Isopropyl-beta-D-thiogalactopyranoside.
ND: not determined.


Supported by UNESCO / MIRCEN network