Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2002 by Universidad Católica de Valparaíso -- Chile
Vol. 5 No. 2, Issue of August 15, 2002
 


Table 2. Catabolic characterization of A. lwoffii RB2.

Substrate

Growth

% Dechlorination*

Phenol

+

n.a.**

3-chlorophenol

-

0

4-chlorophenol

-

6

2,4-dichlorophenol

-

0

Benzoate

+

n.a.

2-chlorobenzoate

-

0

3-chlorobenzoate

-

20

4-chlorobenzoate

-

0

2,4-dichlorobenzoate

-

0

3,5-dichlorobenzoate

-

0

Guaiacol

+

n.a.

4-chloroguaiacol

-

28

5-chloroguaiacol

-

95

6-chloroguaiacol

-

0

3,5-dichloroguaiacol

-

0

4,5-dichloroguaiacol

-

0

4,6-dichloroguaiacol

-

0

Catechol

-

n.a.

3-chlorocatechol

-

0

4-chlorocatechol

-

10

3,5-dichlorocatechol

-

11


*% Dechlorination: calculated from the stoichiometric theoretical value. Growth tests were carried out in minimal medium liquid cultures with 0.2-2 mM carbon source and incubated for up to 10 days. For dechlorination assays cells were pre-grown on the corresponding non-chlorinated substrate. In the case of chlorocatechols cells were pre-grown on 2 mM benzoate. Dechlorination was determined after 1 day with 1 mM substrate.

**n.a.: non applicable.


Supported by UNESCO / MIRCEN network