Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2002 by Universidad Católica de Valparaíso -- Chile
Vol. 5 No. 2, Issue of August 15, 2002
 


Table 1. Strains and plasmids used in this work.

Strain/plasmid

Relevant phenotype/genotype

Source

R.eutropha JMP134 (pJP4)

2,4-D, 3-CB

DSMZ

R. eutropha JMP222

Smr

H. Knackmuss

P. putida KT2442

Rfr

(de Lorenzo et al. 1990)

E. coli CC118lpir

Rfr

(de Lorenzo et al. 1990)

E. coli HB101 (pRK600)

Cmr, tra+

(Herrero et al. 1990)

A. lwoffii RB2

Gu

this work

pUT

Kmr, Apr

(de Lorenzo et al. 1990)

pCNB5

Kmr, Apr, lacIq/Ptrc

(de Lorenzo et al. 1993)

pITFD, pCNB5 derivative

Kmr, Apr, lacIq /Ptrc tfdICDEF

this work

pDTFD, pUT derivative

Kmr, Apr, tfdR/Ptfd-I tfdICDEF

this work

pTFD, pUT derivative

Kmr, Apr, Ptfd-I tfdICDEF

this work


2,4-D, 3-CB, Gu: able to grow on 2,4-dichlorophenoxyacetate, 3-chlorobenzoate, or guaiacol; tra+: IncP transference functions; tfd: catabolic genes from pJP4; lacIq: lac repressor mutant; Ptrc: lac/trp hybrid promoter; tfdR: regulatory gene of pJP4; Ptfd-I: promoter region for tfdICDEF cluster; Ap: ampicillin; Km: kanamycin; Rf: rifampycin; Cm: cloramphenicol; Sm: streptomycin. DSMZ: Deutsche Sammlung von Mikroorganismen und Zellkulturen.

Supported by UNESCO / MIRCEN network