Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2002 by Universidad Católica de Valparaíso -- Chile
Vol. 5 No. 1, Issue of April 15, 2002
 


Table 3. Effect of KCl concentration on the growth of P. chrysogenum transformants obtained by two methods.

The average diameter of colonies (mm)

The concentration of KCl (mol/L)

0

0.5

0.9

1.3

1.7

2.1

2.5

2.9

3.3

3.7

4.1

Control           

>7.5

>7.5

5-7.5

<5

-

-

-

-

-

-

-

Transformants-p

-

-

<5

5-7.5

>7.5

>7.5

>7.5

>7.5

>7.5

>7.5

>7.5

Transformants-a

>7.5

>7.5

5-7.5

<5

-

-

-

-

-

-

-

Note: the diameter of colony >7.5mm, good growth; 7.5mm>the diameter of colony<5mm , poor growth; -, no growth; Transformants-p: P. chrysogenum transformants by transformation of protoplasts with PEG; Transformants-a: P. chrysogenum transformants using A. tumefaciens; twenty strains were selected from control, transformants-p, transformants-a, respectively.

Supported by UNESCO / MIRCEN network