Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2001 by Universidad Católica de Valparaíso -- Chile
Vol. 4 No. 2, Issue of August 15, 2001
 

Table 1. Selectable marker genes used in wheat transformation.

Gene

Selective agent

Mode of Action

Reference

pat, bar

Phosphinothricin, bialaphos

Inhibits glutamine synthase

Vasil et al. 1992
Vasil et al. 1993
Weeks et al. 1993

nptII

Kanamycin, G418, paromomycin

Binds 30S ribosomal subunit, inhibits translation

Nehra et al. 1994
Cheng et al. 1997
Witrzens et al. 1998

hpt

Hygromycin

Binds 30S ribosomal subunit, inhibits translation

Ortiz et al. 1996

EPSPS
oxidoreductase

Glyphosate

Inhibits aromatic acid biosynthesis (EPSPS)

Zhou et al. 1995

Glyphosate oxidoreductase (GOX)

Glyphosate

Degradation of glyphosate into aminomethyl phosphonic acid

Zhou et al. 1995

Mannose-6-phosphate
Isomerase

Mannose

Ability to grow on mannose as a sole carbon source

Hansen and Wright, 1999

Cyanamide hydratase (Cah)

Cyanamide

Ability to grow on cyanamide containing media, converts cyanamide into urea

Weeks, 2000

Abbreviations:
bar and pat: encodes a phosphinothricin acetyl transferase
nptII: neomycin phosphotransferase II
hpt: hygromycin phosphotransferase
EPSPS: 5-enolpyruvyl-shikimate-3-phosphate synthase


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