Figure 2. Comparative profile expression
of differentially expressed transcripts in stressed and irrigated peanuts.
(A) Total RNA isolated from
stressed (S) and irrigated (I) peanut leaf tissue corresponding to the
samples used in the differential display experiments were subjected
to slot blot analysis using the re-amplified DDRT fragment (named as
PTRD-1, PTRD-2 etc) as a probe.
(B) Quantitative detection of differentially expressed transcripts by
estimating relative intensity of radioactivity found with each band
in slot blot analysis shown in Figure 2A. Similar results were obtained
in two independent slot blot experiments.