Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2001 by Universidad Católica de Valparaíso -- Chile
Vol. 4 No. 2, Issue of August 15, 2001
 



Indicate down-regulated (suppressed) transcripts following water stress.
Indicate up-regulated (induced) transcripts following water stress.
Indicate new (activated) transcripts following water stress.
(A) Primer combination P2/T7 was effective to identify down-regulated transcripts.
(B) P2/T4.
(C) P2/T8 were good in identifying both down- and up-regulated transcripts.
 

Figure 1. DDRT profile of stressed and irrigated peanut plants. Differentially displayed bands in DDRT-PCR of total RNA extracted from water stress (S) and irrigated (I) peanut (Arachis hypogaea L. cv. Florunner) plants using different primer combinations (P indicate arbitrary and T indicate anchored primer, for details see Table 1). Two lanes (a, b) under S and I reflect two different dilutions of cDNA used in DDRT reaction.


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